Recombinant Enhanced Green Fluorescent Protein Val2-Lys239

Enhanced Green Fluorescent Protein (EGFP) is a widely used fluorescent protein marker in molecular and cell biology research. As an engineered variant of the original Green Fluorescent Protein (GFP) isolated from the jellyfish Aequorea victoria, EGFP features optimized codon usage and improved folding efficiency, resulting in brighter fluorescence and greater photostability compared to wild-type GFP. This recombinant protein emits bright green fluorescence when exposed to blue or UV light, making it an invaluable tool for tracking gene expression, protein localization, and cellular dynamics in living systems. EGFP maintains its fluorescent properties across a wide pH range and demonstrates exceptional resistance to photobleaching, enabling long-term visualization experiments. Its non-invasive nature and ability to function without additional substrates or cofactors has revolutionized live-cell imaging techniques.